Clonal amplification of Fasciola hepatica in Galba truncatula: within and between isolate variation of triclabendazole-susceptible and -resistant clones

An amino acid substitution in Fasciola hepatica P-glycoprotein from triclabendazole-resistant and triclabendazole-susceptible populations.

Control of fasciolosis is threatened by the development of anthelmintic resistance. Enhanced triclabendazole (TCBZ) efflux by ABC transporters such as P-glycoprotein (Pgp) has been implicated in this process. A putative full length cDNA coding for a Pgp expressed in adult Fasciola hepatica has been constructed and used to design a primer set capable of amplifying a region encoding part of the s...

Lack of reversion in triclabendazole-resistant Fasciola hepatica.

RESISTANCE to triclabendazole in field isolates of the liver fluke Fasciola hepatica in sheep was described for the first time by Overend and Bowen (1995) in Australia. In Europe, resistance of F hepatica in sheep to triclabendazole has been reported in Ireland (Lane 1998), Scotland (Mitchell and others 1998), Wales (Thomas and others 2000) and the Netherlands (Moll and others 2000). On the Dut...

Study of surface carbohydrates in Galba truncatula tissues before and after infection with Fasciola hepatica

The presence and distribution of surface carbohydrates in the tissues of Galba truncatula snails uninfected or after infection with Fasciola hepatica as well as on the surface of the snail-pathogenic larval stages of the parasite were studied by lectin labelling assay. This is an attempt to find similarities that indicate possible mimicry, utilised by the parasite as an evasion strategy in this...

Apparent Triclabendazole-Resistant Human Fasciola hepatica Infection, the Netherlands

1. European Food Safety Authority. EFSA Panel on Animal Health and Welfare; scientifi c opinion on African swine fever. EFSA Journal. 2010;8:1556. http://dx.doi. org/10.2903/j.efsa.20101556 2. Sanchez-Botija C. Reservoirs of ASFV: a study of the ASFV in arthropods by means of the haemadsorption test. Bull Off Int Epizoot. 1963;60:895–9. 3. Boinas FS, Wilson AJ, Hutchings GH, Martins C, Dixon LJ...

Identification of a field isolate of Fasciola hepatica resistant to albendazole and 1 susceptible